CONCLUSIONS: These results suggest that NCI-N87 cell line may be useful in H. O contraste de fase, ao converter espcimes de fase como material vivo em espcimes de amplitude, permitiu aos cientistas ver detalhes em objectos no manchados e/ou vivos com uma clareza e resoluo nunca antes alcanada. In NCI-N87 cell lines, IL-8 level was significantly increased after 24 hour compared to control, and was prominent in the lower well. A tcnica de contraste de fase foi aclamada como o maior avano em microscopia num sculo. TER was measured as 400-500 (omega x cm2) at two or three weeks after cell culture.
RESULTS: A monolayer phenotype was observed in NCI-N87 cell lines by using confocal microscope. IL-8 level was compared in both upper and lower well with the control. Las preparaciones sin teñir y por lo tanto transparentes que no absorben la luz se llama muestras de fases, ya que alteran ligeramente la luz que pasa a través de ellos, por lo general el contraste de fases retarda 1/4 de longitud de onda con respecto a la luz. The IL-8 level was determined by ELISA at 24 hours after the administration of HP60190 and IL-1alpha on NCI-N87 cells. Transepithelial resistance (TER) was measured on NCI-N87 cells seeded on Transwell(R) to identify monolayer polarity two or three times a week after confluency. Esta tcnica de microscopa aprovecha las pequeas. 1 La mayora de los organismos vivos no pueden ser teidos debido a que los colorantes utilizados pueden daar su estructura celular hasta el punto de su muerte.
METHODS: For the identification of epithelial phenotypes at low magnification, cells were observed on a phase-contrast microscope and confocal microscope. El microscopio de contraste de fases permite observar clulas sin colorear y resulta especialmente til para clulas vivas. The present investigation aimed at assessing the usefulness of NCI-N87 cell line as an adequate cellular model to study the pathophysiology of human H.
BACKGROUND/ AIMS: The unavailability of human gastric cell lines representative of the normal gastric epithelial function such as polarized monolayer restricts the application of cell culture system in approaching the field of Helicobacter pylori (H.
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